Isolation and characterization of two genes encoding polygalacturonase-inhibiting protein from Populus deltoides.
Identifieur interne : 003882 ( Main/Exploration ); précédent : 003881; suivant : 003883Isolation and characterization of two genes encoding polygalacturonase-inhibiting protein from Populus deltoides.
Auteurs : Qiang Cheng [République populaire de Chine] ; Youzhi Cao ; Huixin Pan ; Mingxiu Wang ; Minren HuangSource :
- Journal of genetics and genomics = Yi chuan xue bao [ 1673-8527 ] ; 2008.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Facteur de croissance végétal (pharmacologie), Gènes de plante (MeSH), Phylogenèse (MeSH), Populus (effets des médicaments et des substances chimiques), Populus (génétique), Populus (physiologie), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Stress physiologique (génétique), Séquence conservée (MeSH), Séquence d'acides aminés (MeSH), Transduction du signal (génétique).
- MESH :
- composition chimique : Protéines végétales.
- effets des médicaments et des substances chimiques : Populus, Régulation de l'expression des gènes végétaux.
- génétique : ADN complémentaire, Populus, Protéines végétales, Stress physiologique, Transduction du signal.
- métabolisme : Protéines végétales.
- pharmacologie : Facteur de croissance végétal.
- physiologie : Populus.
- Clonage moléculaire, Données de séquences moléculaires, Gènes de plante, Phylogenèse, Séquence conservée, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Cloning, Molecular (MeSH), Conserved Sequence (MeSH), DNA, Complementary (genetics), Gene Expression Regulation, Plant (drug effects), Genes, Plant (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Growth Regulators (pharmacology), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (drug effects), Populus (genetics), Populus (physiology), Signal Transduction (genetics), Stress, Physiological (genetics).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , genetics : DNA, Complementary, Plant Proteins.
- drug effects : Gene Expression Regulation, Plant, Populus.
- genetics : Populus, Signal Transduction, Stress, Physiological.
- chemical , metabolism : Plant Proteins.
- chemical , pharmacology : Plant Growth Regulators.
- physiology : Populus.
- Amino Acid Sequence, Cloning, Molecular, Conserved Sequence, Genes, Plant, Molecular Sequence Data, Phylogeny.
Abstract
Polygalacturonase-inhibiting proteins (PGIPs) are extracellular proteins that belong to the leucine-rich repeat (LRR) protein superfamily. PGIPs inhibit fungal polygalacturonases (PGs) and promote accumulation of oligogalacturonides, which activate plant defense responses. PGIPs play important roles in resistance to infection of pathogens. In this study, reverse transcriptase-polymerase chain reaction (RT-PCR) and RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) were used to isolate the full-length PGIP cDNA from Populus deltoides (GenBank accession no. of PdPGIP2 and PdPGIP4: EF684913 and EF684912). Domain analysis revealed that the deduced amino acid sequences of PdPGIP2 and PdPGIP4 had a typical PGIP topology. Phylogenetic analysis of known PGIPs indicated that the two PdPGIPs were clustered to the defense-related PGIP clade. Using real-time RT-PCR, the expression patterns of the two PdPGIPs following treatment with a fungal pathogen and defense-related signaling molecules were studied. The expression levels of PdPGIP2 and PdPGIP4 were both up-regulated when inoculated with the phytopathogenic fungus Marssonina brunnea. Therefore, it was proposed that the two PGIPs might be involved in the resistance to Marssonina brunnea in P. deltoides.
DOI: 10.1016/S1673-8527(08)60084-3
PubMed: 18937920
Affiliations:
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Data</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Gènes de plante</term><term>Phylogenèse</term><term>Séquence conservée</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polygalacturonase-inhibiting proteins (PGIPs) are extracellular proteins that belong to the leucine-rich repeat (LRR) protein superfamily. PGIPs inhibit fungal polygalacturonases (PGs) and promote accumulation of oligogalacturonides, which activate plant defense responses. PGIPs play important roles in resistance to infection of pathogens. In this study, reverse transcriptase-polymerase chain reaction (RT-PCR) and RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) were used to isolate the full-length PGIP cDNA from Populus deltoides (GenBank accession no. of PdPGIP2 and PdPGIP4: EF684913 and EF684912). Domain analysis revealed that the deduced amino acid sequences of PdPGIP2 and PdPGIP4 had a typical PGIP topology. Phylogenetic analysis of known PGIPs indicated that the two PdPGIPs were clustered to the defense-related PGIP clade. Using real-time RT-PCR, the expression patterns of the two PdPGIPs following treatment with a fungal pathogen and defense-related signaling molecules were studied. The expression levels of PdPGIP2 and PdPGIP4 were both up-regulated when inoculated with the phytopathogenic fungus Marssonina brunnea. Therefore, it was proposed that the two PGIPs might be involved in the resistance to Marssonina brunnea in P. deltoides.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18937920</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>09</Day></DateCompleted><DateRevised><Year>2008</Year><Month>10</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1673-8527</ISSN><JournalIssue CitedMedium="Internet"><Volume>35</Volume><Issue>10</Issue><PubDate><Year>2008</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of genetics and genomics = Yi chuan xue bao</Title><ISOAbbreviation>J Genet Genomics</ISOAbbreviation></Journal><ArticleTitle>Isolation and characterization of two genes encoding polygalacturonase-inhibiting protein from Populus deltoides.</ArticleTitle><Pagination><MedlinePgn>631-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/S1673-8527(08)60084-3</ELocationID><Abstract><AbstractText>Polygalacturonase-inhibiting proteins (PGIPs) are extracellular proteins that belong to the leucine-rich repeat (LRR) protein superfamily. PGIPs inhibit fungal polygalacturonases (PGs) and promote accumulation of oligogalacturonides, which activate plant defense responses. PGIPs play important roles in resistance to infection of pathogens. In this study, reverse transcriptase-polymerase chain reaction (RT-PCR) and RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) were used to isolate the full-length PGIP cDNA from Populus deltoides (GenBank accession no. of PdPGIP2 and PdPGIP4: EF684913 and EF684912). Domain analysis revealed that the deduced amino acid sequences of PdPGIP2 and PdPGIP4 had a typical PGIP topology. Phylogenetic analysis of known PGIPs indicated that the two PdPGIPs were clustered to the defense-related PGIP clade. Using real-time RT-PCR, the expression patterns of the two PdPGIPs following treatment with a fungal pathogen and defense-related signaling molecules were studied. The expression levels of PdPGIP2 and PdPGIP4 were both up-regulated when inoculated with the phytopathogenic fungus Marssonina brunnea. Therefore, it was proposed that the two PGIPs might be involved in the resistance to Marssonina brunnea in P. deltoides.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Key Laboratory of Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Youzhi</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Huixin</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Mingxiu</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Minren</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal 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Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010937" 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MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>02</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2008</Year><Month>07</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>07</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>10</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>4</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18937920</ArticleId><ArticleId IdType="pii">S1673-8527(08)60084-3</ArticleId><ArticleId IdType="doi">10.1016/S1673-8527(08)60084-3</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Cao, Youzhi" sort="Cao, Youzhi" uniqKey="Cao Y" first="Youzhi" last="Cao">Youzhi Cao</name><name sortKey="Huang, Minren" sort="Huang, Minren" uniqKey="Huang M" first="Minren" last="Huang">Minren Huang</name><name sortKey="Pan, Huixin" sort="Pan, Huixin" uniqKey="Pan H" first="Huixin" last="Pan">Huixin Pan</name><name sortKey="Wang, Mingxiu" sort="Wang, Mingxiu" uniqKey="Wang M" first="Mingxiu" last="Wang">Mingxiu Wang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Cheng, Qiang" sort="Cheng, Qiang" uniqKey="Cheng Q" first="Qiang" last="Cheng">Qiang Cheng</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document 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